19+ neb q5 tm calculator

Optimal annealing temperatures for Q5 Hot Start High-Fidelity DNA Polymerase tend to be higher than for other PCR polymerases. I just have a question about the NEB Tm calculator as I plan on using their Q5.

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The NEB Tm Calculator should be used to.

. The NEB Tm calculator is intended for use in estimating the optimal annealing temperature for PCR with NEB polymerases Tm values are calculated using thermodynamic data from Santa. For POST requests the input is expected to be in serialized JSON format including an HTTP content-type header. How to use the Tm calculator Amplification of a variety of human genomic amplicons from low to high GC content using Q5 High-Fidelity DNA Polymerase.

Unlike other calculators the NEB Tm Calculator takes buffer components that affect melting temperatures into consideration when calculating the best annealing temperature.

To Use The Neb Tm Calculator Is It The Addition Of The Forward And Reverse Primer Concentration That Is Required Or Just One Of Them Researchgate

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